Fig. 4.

Cumulus cell expression of H19 in women with PCOS versus controls (male/tubal factor). A total of 39 women undergoing in vitro fertilization (29 non-PCOS women undergoing IVF for male factor infertility and 10 women with PCOS) were evaluated. At the time of oocyte retrieval, cumulus cells were collected, total RNA was isolated, and cDNA was synthesized by reverse transcription. RNA levels were analyzed using quantitative real-time PCR. H19 RNA levels were normalized to those of β-actin and presented as relative expression levels using the comparative CT method. Box plot demonstrates increased relative H19 expression (2.8-fold) in cumulus cells of PCOS patients undergoing IVF, compared to non-PCOS patients (p < 0.05)