FIG. 5.

RT-PCR analysis to confirm differential gene expression. RT-PCR was performed with both aerobic (A) and anaerobic (An) RNA with primers specific for each product. Product numbers correspond to those given in Table 1. Relative quantities of each RT-PCR product amplified from both aerobic and anaerobic RNA were analyzed after normalization to the internal control. Paired control reactions lacking RT showed no products derived from genomic DNA.