Table 2.
Identification of alkaloid-related compounds and key genes in Dendrobium spp.
| Species | Sequencing platform or technique | Classification | Annotated genes | Ref. |
|---|---|---|---|---|
| D. nobile | Molecular Cloning | Pyrrolidine alkaloid | TR1 | Chen et al., 2013 |
| D. nobile | Molecular Cloning | Pyrrolidine alkaloid | TR2 | Cheng et al., 2013 |
| D. officinale | Roche 454 GS FLX Titanium | terpenoid indole alkaloids | AACT,HMGS,HMGR,MVK,PMK, MVD,IPI,DXS,DXR,CMS,CMK, MCS,HDS,FPS,TSB,TDC,G10H, SCS,STR,10-HGO | Guo et al., 2013 |
| D. officinale | Illumina Hiseq 2000 and PacBio Sequel | Monoterpene indole alkaloids | AACT,HMGS,HMGR, MVK, PMK, MVD,IPI, DXS,DXR,CMS,CMK, MCS,HDS,FPS, PNAE | Yan et al., 2015 |
| D. officinale | Molecular Cloning | Precursor of terpenoid | DXS,DXR | Fan et al., 2016 |
| D. catenatum | Illumina HiSeq 2000 | Precursor of terpenoid | TPS-a, TPS-b, TPS-e/f, TPS-c, TPS-g | Zhang et al., 2016a |
| D. officinale | Illumina Hiseq 2000 | Indole alkaloids, isoquinoline alkaloid, tropane, piperidine, and pyridine alkaloid | Not stated | Zhang et al., 2016b |
| D. nobile | Agilent Bioanalyzer 2,100 | Sesquiterpene alkaloids | AACT,HMGS,HMGR,MK,PMK, MVD,GPPS,FPS,TPS21,CYP71D55,CYP735A,CYP71D10,CYP94C1, METTL23,ATX4,AAT2,DAT, BCAT2 | Li et al., 2017b |
| D. officinale | Agilent Bioanalyzer 2,100 | terpenoid indole alkaloids | AACT,HMGS,HMGR,MVK,PMK, MVD,IPI,DXS,DXR,CMS,CMK, MCS,HDS,FPS,TSB,TDC,STR | Shen et al., 2017 |
| D. officinale | Molecular Cloning | Precursor of terpenoid | MYC2 | Zhu et al., 2017 |
| D. huoshanense | Illumina Hiseq 2,500 | terpenoid indole alkaloids | FPS,AACT,HMGS,HMGR,MVK, PMK,MVD,IPI,DXS,DXR,CMS, CMK,MCS,TDS,TSB,HDR | Yuan et al., 2018 |
| D. officinale | Illumina Hiseq 4,000 | Sesquiterpene alkaloids | AACT,HMGS,HMGR,MVK,PMK, MVD,DXS,DXR,MCT,CMK,MDS, HDS,HDR,FPS,IPI | Chen et al., 2019 |
| D. officinale | Molecular Cloning | Monoterpene indole alkaloids | STR | Zhu et al., 2020 |
| D. officinale | Genome-wide identification | Precursor of indole alkaloids | GAD,HDC,TYDC,TDC | Zhang et al., 2020 |
| D. officinale | Genome-wide identification | Precursor of terpenoid | TPS-a, TPS-b, TPS-c, TPS-e/f | Yu et al., 2020 |
| D. chrysotoxum | MGISEQ-2000 and PacBio Sequel | Precursor of terpenoid | TPS-a, TPS-b, TPS-c, TPS-e/f, TPS-g | Zhang et al., 2021c |
| D. officinale | Illumina Hiseq 4,000 and PacBio Sequel II | Sesquiterpene alkaloids, isoquinoline alkaloid, pyrrolidine alkaloid, terpenoid indole alkaloids | AOC, AAT, TAT, TDC, CNMT, N4OMT,TR1,PNAE,HMGS,HMGR,MVK,PMK,MVD,DXS,DXR, CMS,CMK,MCS,PNAE,SDR,AAE, TPS21,FDFT1,SQLE,FLDH, CYP71D55 | Niu et al., 2021 |
| D. officinale | BGIseq 500 | Monoterpene indole alkaloids | TS,TDC,STR,HDR,GPPS,GES, G10H,UGT8,7-DLH,LAMT,SG, GS,PNAE,VS,AACT,HMGS, HMGR, MVK,PMK,MVD,IPI, DXD,DXR,CMS,CMK,MCS,HDS, SCS | Wang et al., 2021 |
| D. officinale | Illumina HiSeq 4,000 | Precursor of terpenoid | TPS-a, TPS-b, TPS-c, TPS-e/f | Li et al., 2021 |