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. 2022 May 6;13:844811. doi: 10.3389/fmicb.2022.844811

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Copyright © 2022 Mohamed, Marusich, Afanasev and Leonov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The MIC₅₀ (IC₅₀ value in the figure legends) of K. pneumoniae strains treated with AWME3 from the larvae fat compared to the doxycycline (DOX) as a positive control. The MIC₅₀ values were calculated based on the turbidimetric assay data and compared to the positive control (DOX). The planktonic bacteria turbidity was assessed for (A,B) K. pneumoniae ATCC BAA-2473; (C,D) K. pneumoniae KPM9; (E,F) K. pneumoniae KPi1627 strains at 12 and 24 h incubation with (A,C,E) DOX; (B,D,F) AWME3. The MIC₅₀ values were calculated using the non-linear regression mode of Graph pad Prism 7 (Graph Pad Software Inc., San Diego, CA, United States). The (IC₅₀)MIC₅₀ values are the average of three independent experiments ± standard deviation error mean (SEM).