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. 2022 May 6;13:844811. doi: 10.3389/fmicb.2022.844811

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Copyright © 2022 Mohamed, Marusich, Afanasev and Leonov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytotoxicity of AWME3 fat on HEK-293 cell lines. Cells were treated with serial of AWME3 dilutions for 24 h. Then, viability of AWME3 treated-cells was measured using MTT assay (A,B). Where, (A) cell lines were treated with AWME3 for 24 h (X-axis: log concentrations of AWME3 extract from 0 to 1,000 (μg/mL) and Y-axis: the percentage of normalized absorbance). (B) The of average OD₅₇₀ of the HEK-293 cell line treated with AWME3 for 24 h (Y-axis: the normalized absorbance of HEK-293 at OD₅₇₀), X-axis: AWME3 concentrations from 0 to 1,000 μg/mL. The IC₅₀ values were calculated using the non-linear regression mode of Graph pad Prism7 (Graph Pad Software Inc., San Diego, CA, United States). All Results are the mean (±SEM) from three independent experiments performed in triplicates (n = 8). Statistical values are indicated.