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Summary of half times for PtdIns(4,5)P2 synthesis and breakdown. The diagram represents some important steps in phosphoinositide metabolism—the PI cycle—and the measured half times for segments we could study in living tsA201 cells at room temperature as explained in the text. Everything might be three to four times faster at 37°C. DAG, diacylglycerol; PI, PtdIns; PI(4)P, PtdIns(4)P; PI(4,5)P2, PtdIns(4,5)P2.
