Skip to main content
. 2022 May 6;13:849930. doi: 10.3389/fpls.2022.849930

Figure 3.

Figure 3

Characterization of T-circles composed of two different T-DNAs. (A) Two T-DNAs can end-join to each other in either of two configurations: “Head-to-tail” that will produce 3.9 and 0.7 kbp DrdI fragments (left) or “head-to-head” and “tail-to-tail” that will produce 2.2 and 2.4 kbp DrdI fragments (right). (B) 50 T-circles conferring resistance to both kanamycin and tetracycline following co-agroinfiltration with Agrobacterium tumefaciens EHA105 containing the KAN-ORI and TET-ORI T-DNA binary vectors. Each plasmid is shown undigested (U) and DrdI digested (D). Eighteen unique sequenced T-circles are marked in red (T-circles #19, #20, #32, #38, #39, #43, #44, and #50 are duplicates).