Figure 5.

Transient expression of GUS activity in N. benthamiana leaves agroinfiltrated with Agrobacterium containing a wild-type VirD2 gene (left panels) or the virD2 ω mutant (right panel). Agrobacterium tumefaciens containing a wild-type virD2 gene, the AMP-ORI T-circle binary vector, and the T-DNA binary vector pBISN1 or a similar strain with the ω mutant virD2 gene were infiltrated at the indicated concentration. Discs cut from leaves after 4 days were stained with X-gluc, cleared with 70% ethanol, and the relative staining intensity calculated using ImageJ software. cfu, colony forming units.