Table 4.

Sequenced T-DNA junctions of complex T-circles.

Sample no.	Strain	Construct	RB*	Microhomologya	Filler DNAb	Microhomologyc	LB*
Nicotiana benthamiana
#002–23	EHA105	TET-ORI	Readthrough >380 bp	NA	NA	NA	NA
#003–50	EHA105	TET-ORI	Precise	2 (GA)	236 bp binary +9 bp internal T-DNA sequence	0	−72
#003–61	EHA105	TET-ORI	−1	2 (TG)	>486 bp binary sequence	NA	NA
#003–62	EHA105	TET-ORI	Precise	4 (TTGA)	>489 bp binary sequence	NA	NA
#003–64	EHA105	TET-ORI	Precise +1	0	>202 bp internal T-DNA sequence	NA	NA
#008–73	EHA105	TET-ORI	Precise	1 (A)	290 bp plant DNA	6 (TCAGGC)	−11
#009–12	EHA105	TET-ORI	Precise +4	5 (CAGGC)	>585 bp binary sequence	NA	NA
#050–14	EHA105	AMP-ORI	Readthrough 151 bp	NA	0	0	−215
#050–23	EHA105	AMP-ORI	Precise	0	>431 bp binary sequence	NA	NA
#052–9	EHA105	AMP-ORI	Precise+1	1 (C)	284 bp binary sequence	3 (AGC)	−456
#052–17	EHA105	AMP-ORI	Readthrough 224 bp	NA	0	0	−237
#052–18	EHA105	AMP-ORI	−21	4 (TTCA)	188 bp plant DNA	0	−239
#052–22	EHA105	AMP-ORI	Precise +1	0	>406 bp Ti plasmid sequence	NA	NA
#052–28	EHA105	AMP-ORI	Precise	0	84 bp T-DNA sequence	3 (GCT)	−436
#052–35	EHA105	AMP-ORI	Precise	0	>484 bp binary sequence	NA	NA
#052–44	EHA105	AMP-ORI	Precise+1	1 (C)	375 bp Agrobacterium chromosomal DNA	3(CGA)	−317
#052–45	EHA105	AMP-ORI	Readthrough 129 bp	NA	0	3 (TTT)	−714
#052–55	EHA105	AMP-ORI	Precise	2 (GA)	74 bp internal T-DNA sequence	2(GG)	−683
#052–60	EHA105	AMP-ORI	Precise +2	2 (CA)	>560 bp binary sequence	NA	NA
#052–63	EHA105	AMP-ORI	Readthrough 192 bp	NA	0	0	−265
#052–66	EHA105	AMP-ORI	Precise	0	>799 bp Ti plasmid sequence	NA	NA
VirD2ω
#005–8	At1959	TET-ORI	−11	0	234 bp binary sequence	4 (AACA)	−46
Arabidopsis Col-0
#021–4	EHA105	AMP-ORI	Readthrough >461 bp	NA	NA	NA	NA

NA, not available.

^*Right border (RB) and left border (LB) numerical values represent the position in the DNA relative to the precise end.

^aMicrohomology between RB and filler DNA.

^bFiller DNA is defined here as any DNA sequence not part of the contiguous T-DNA.

^cMicrohomology between filler and LB.