Table 5.
Summary of properties of T-circles from Nicotiana benthamiana generated by pE4636.
| Number | Precise RB | Precise LBa | Microhomology at RB region | Microhomology at LB region | Filler DNA | Major rearrangements |
|---|---|---|---|---|---|---|
| 42 | 23 (55%) | 0 (out of 39; 0%) | 13 (31%) | 7 (17%) | 2 (plant; 5%) 3 (pAtC58; 7%) 4 (binary; 10%) 4 (few bp; 10%) |
Binary vector or T-DNA reverse complement or other unknown rearrangement (15; 36%) |
a
In some instances, Sanger sequencing did not extend far enough to obtain a LB region sequence if there were a large filler. For some plasmids, Wide-seq analysis revealed the entire T-circle sequence.