Table 6.
T-DNA junctions of T-circles from Agrobacterium benthamiana using virD2 ω mutant Agrobacterium strains.
| Number characterized | Strain | Construct | Precise RB | Precise LBa | RB Microhomology | LB Microhomologya | Filler DNA |
|---|---|---|---|---|---|---|---|
| 6 | At1959 | TET-ORI or AMP-ORI |
6 (100%) | 3 (50%) | 1 (17%) | 0 | 1 (1 bp, A) |
| 18 | At2332 | pE4636 | 18 (100%) | 5 (of 12; 42%) | 5 (28%) | 2 (of 13; 15%) | 6 (pAtC58) 3 (linear chromosome) |
a
In some instances, Sanger sequencing did not extend far enough to obtain a LB region sequence if there were a large filler. For some plasmids, Wide-seq analysis revealed the entire T-circle sequence.