TABLE 4.

gyrB PCR primers in the differentiation of B. cereus, B. thuringiensis, B. anthracis, and B. mycoides

Specificity	Primer	Oligonucleotide sequence	No. of bpa
Universal primersb	UP-1	GAA GTC ATC ATG ACC GTT CTG CAY GCN GGN GGN AAR TTY GA	41
	UP-2r	AGC AGG ATA CGG ATG TGC GAG CCR TCN ACR TCN GCR TCN GTC AT	44
B. cereus	BC1	ATT GGT GAC ACC GAT CAA ACA	21
	BC2r	TCA TAC GTA TGG ATG TTA TTC	21
B. thuringiensis	BT1	ATC GGT GAT ACA GAT AAG ACT	21
	BT2r	CCT TCA TAC GTA TGA ATA TTA TTT	24
B. anthracis	BA1	AAT CGT AAT ATT AAA CTG ACG	21
	BA2r	CCT TCA TAC GTG TGA ATG TTG	21

^abp, base pairs. 

^bUniversal primers were as described by Yamamoto and Harayama (50).