TABLE 5.
Specificity of gyrB PCR primers in the differentiation of B. cereus, B. thuringiensis, B. anthracis, and B. mycoides
| Bacterial strain and serotypea | Amplification of a specific PCR fragmentb that identified the strain as:
|
||
|---|---|---|---|
| B. cereus | B. thuringiensis | B. anthracis | |
| B. cereus | |||
| JCM 2152T | + | − | − |
| H1 | + | − | − |
| H2 | + | − | − |
| H3 | + | − | − |
| H4 | + | − | − |
| H5 | − | − | + |
| H6 | − | − | + |
| H7 | − | − | + |
| H8 | + | − | − |
| H9 | + | − | − |
| H10 | + | − | − |
| H11 | + | − | − |
| H12 | + | − | − |
| H13 | + | − | − |
| H14 | + | − | − |
| H15 | + | − | − |
| H16 | − | + | − |
| H17 | − | − | + |
| H18 | + | − | − |
| B. thuringiensis | |||
| berliner (IAM 12077T) | − | + | − |
| kurstaki (HD-1) | + | − | − |
| galleriae | − | + | − |
| aizawai | + | − | − |
| israelensis | − | + | − |
| B. anthracis Pasteur #2H | − | − | + |
| B. mycoides ATCC 6462T | − | − | − |
a
JCM, Japan Collection of Microorganisms; IAM, Institute of Applied Microorganisms; ATCC, American Type Culture Collection; and T, type strain.
b
PCR primer sets BC1-BC2r, BT1-BT2r, and BA1-BA2r were used to amplify B. cereus-, B. thuringiensis-, and B. anthracis-specific fragments, respectively.