TABLE 7.
Comparison of gyrB PCR and 16S rDNA hybridization probe techniques in the differentiation of B. cereus and B. thuringiensis
| Bacterial strain or serotype | Hybridization signal positive for:
|
Amplification of gyrB PCR fragment that is specific to:
|
|||
|---|---|---|---|---|---|
| B. cereusa | B. thurin-giensisb | B. cereusc | B. thurin-giensisd | B. an-thracise | |
| B. cereus | |||||
| JCM 2152T | + | − | + | − | − |
| H5 | − | − | − | − | + |
| H6 | − | − | − | − | + |
| H7 | − | − | − | − | + |
| H16 | + | − | − | + | − |
| H17 | − | − | − | − | + |
| Nagoya 126f | − | + | − | + | − |
| Nagoya 127f | − | + | − | + | − |
| B. thuringiensis | |||||
| berliner (IAM 12077T) | − | + | − | + | − |
| kurstaki (HD-1) | + | − | + | − | − |
| galleriae | − | + | − | + | − |
| aizawai | + | − | + | − | − |
| israelensis | − | + | − | + | − |
| B. anthracis Pasteur #2H | − | − | − | − | + |
| B. mycoides ATCC 6462T | + | − | − | − | − |