Fig. 1.

Effect of sensory neurone-transient receptor potential cation channel subfamily V member 4 (TRPV4) on acute itch induced by pruritogens evoked acute scratching behaviours that were significantly attenuated in Trpv4 conditional knockout (cKO) vs wild-type mice (WTs) in both males (A) and females (B). Histamine, compound 48/80, and 5-hydroxtryptamine (5-HT), but not Ser-Leu-Ile-Gly-Arg-Leu-NH2 (SLIGRL), evoked acute scratching behaviours that were significantly attenuated in Trpv4 conditional knockout (cKO) vs wild-type mice (WTs) in both sexes. Chloroquine (CQ)-induced itch was significantly reduced in female, but not male, Trpv4 cKO mice. *p < 0.05, **p < 0.01, and ***p < 0.001 vs WT, 2-tail t-test. Group size is indicated on the bars.