TABLE 6.
Strain | % Inhibition of sucrose uptake by unlabeled disaccharidesb
|
|||
---|---|---|---|---|
Sucrose | Trehalose | Maltose | Cellobiose | |
S. meliloti 444c | 95c | 90c | 91c | 92c |
R. leguminosarum | ||||
bv. phaseoli H132d | 96d | 91d | 90d | 88d |
bv. trifolii | ||||
T22 | 96 | 98 | 98 | 68 |
T8S | 97 | 96 | 27 | 68 |
T17S | 98 | 64 | 20 | 19 |
Cultures were grown for 24 h in mannitol-aspartate minimal medium containing 1 mM sucrose plus NaCl at the following concentrations: S. meliloti 444, 0.5 M; R. leguminosarum bv. phaseoli H132, 0.3 M; R. leguminosarum bv. trifolii strains, 0.7 M.
Percentages of inhibition of sucrose uptake were measured after a 5-min incubation in the presence of 0.5 mM [14C]sucrose (23.3 GBq/mmol) plus 25 mM of the indicated unlabeled analog. Uninhibited transport rates were 28 nmol mg of protein−1 for both S. meliloti 444 and R. leguminosarum bv. phaseoli H132, and 17, 27, and 11 nmol mg of protein−1 for R. leguminosarum bv. trifolii strains T22, T8S, and T17S, respectively.
Similar percentages of inhibition were observed in S. meliloti SU47, M5N1, and 2009.
Similar percentages of inhibition were observed in R. leguminosarum bv. phaseoli p12S and p15S.