2× Disaggregation buffer

Reagent	Initial concentration	Final concentration	Amount
HEPES-KOH pH 7.3	0.5 M	40 mM	8 mL
KCl	2 M	300 mM	15 mL
MgCl2	0.5 M	5 mM	1 mL
Triton X-100	100%	0.02%	20 μL
ddH2O	n/a	n/a	56 mL
Total	n/a	n/a	100 mL

This reagent can be stored at 4°C or room temperature for up to one year. Filter buffer and avoid RNase contamination.