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Pab1 condensate dispersal reaction samples
| Reaction | 2 μM Pab1 condensate (μL) | 0.1 M ATP (μL) | 5 μM RNA (μL) | 1× disaggregation buffer (μL) | SUPERase-in (μL) | CK/CP mix (μL) | Hsp104/Ssa2/Sis1 mix (μL) |
|---|---|---|---|---|---|---|---|
| 1 | 3 | 0 | 0.6 | 7.1 | 0.3 | 1 | 3 |
| 2 | 3 | 0.75 | 0.6 | 6.35 | 0.3 | 1 | 3 |
| Final conc. | “0.4 μM” | 5 mM | 0.2 μM | n/a | 2% | 1 μM/8 mM | 0.1/1/0.5 μM |
