REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
Subcloning Efficiency™ DH5α Competent Cells	Thermo Fisher Scientific	Cat#18265017
BL21(DE3) Competent Cells	MilliporeSigma	Cat#69450
Chemicals, peptides, and recombinant proteins
SUPERase•In™ RNase Inhibitor	Thermo Fisher Scientific	Cat#AM2694
Creatine phosphokinase (CK)	MilliporeSigma	Cat#C3755
Creatine phosphate (CP)	GoldBio	Cat#C-323
Protease Inhibitor Cocktail Set IV	MilliporeSigma	Cat#539136
Pierce™ Protease Inhibitor Tablets, EDTA-free	Thermo Fisher Scientific	Cat#A32965
Adenosine-5′-Triphosphate (ATP)	GoldBio	Cat#A-081-1
Bovine serum albumin (BSA)	MilliporeSigma	Cat#12659
Deposited data
Pab1 dispersal example data	This paper	Mendeley Data: https://doi.org/10.17632/4vzph2f83y.1
Oligonucleotides
Fluorescein-A19 RNA: 5′ 6-FAM-AAAAAAAAAA AAAAAAAAA	IDT	N/A
Recombinant DNA
Plasmid: 8×His-(TevC)-Pab1 in pET28a backbone	Riback et al. (2017)	pJAR006
Plasmid: 6×His-(SUMO)-Ssa2 in pET28a backbone	Yoo et al. (2022)	pHY024
Plasmid: 6×His-(SUMO)-Hsp104 in pET28a backbone	Yoo et al. (2022)	pEP055
Plasmid: 6×His-(SUMO)-Sis1 in pET28a backbone	Yoo et al. (2022)	pEP057
Software and algorithms
RStudio ver. 1.2.1335	RStudio Team, 2018	http://www.rstudio.com/
SparkControl™ ver.	Tecan	N/A
Custom R (version 3.5.2) script for example data processing, analysis, and quantification	This paper	Mendeley Data: http://doi.org/10.17632/4vzph2f83y.1
Other
HisTrap FF Column	Cytiva	Cat#17525501
HiTrap Heparin HP Column	Cytiva	Cat#17040701
HiTrap Q HP Column	Cytiva	Cat#17115401
Superose 6 10/300 GL Column	Cytiva	Cat#29091596
SnakeSkin Dialysis Tubing, 10K MWCO, 22 mm	Thermo Fisher Scientific	Cat#68100
NBS™ 384 well microplates (black; flat bottom) for anisotropy assays	Corning	Cat#CLS3575
Nunc™ Sealing Tapes for microplates	Thermo Fisher Scientific	Cat#235307