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. 2022 May 13;53:102329. doi: 10.1016/j.redox.2022.102329

Table 1.

Primers used for RT-PCR and semi-quantitative real time PCR.

Primer Sense Antisense
SOD2 CDS 5′-TCCACTGCAAGGAACAACAG-3′ 5′-CGTGGTTTACTTTTTGCAAGC-3′
SOD2 3′UTR-A 5′-ATAATGCTGGGGTGAGCAAC-3′ 5′-GCTGAGGTGGGACAATCACT-3′
SOD2 3′UTR-B 5′-TGTGTATGCATGCTTGTGGA-3′ 5′CCACCTTGCCCGTCTATTTA-3′
ATF4 5′- TGTCCTCCACTCCAGATCAT 5′-GGCTCATACAGATGCCACTATC-3′
ELAVL1 5′-CGCAGAGATTCAGGTTCTCC-3′ 5′-CCAAACCCTTTGCACTTGTT-3′
PTGS2 CDS 5′- CTGGCGCTCAGCCATACAG-3′ 5′-CCGGGTACAATCGCACTTAT-3′
GAPDH 5′-TGCACCACCAACTGCTTAGC-3′ 5′-GGCATGGACTGTGGTCATGAG-3′
Housekeeping genes for semiquantitative real-time RT-PCR:
GAPDH 5′-GAGTCAACGGATTTGGTCGT-3′ 5′-TTGATTTTGGAGGGATCTCG-3′
18S 5′-AGAAACGGCTACCACATCCA-3′ 5′- CACCAGACTTGCCCTCCA-3′
HPRT1 5′-TGACCTTGATTTATTTTGCATACC-3′ 5′-CGAGCAAGACGTTCAGTCCT-3′
TBP 5′-TTGGGTTTTCCAGCTAAGTTCT-3′ 5′-CCAGGAAATAACTCTGGCTCA-3′