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. 2022 May 13;53:102329. doi: 10.1016/j.redox.2022.102329

Table 2.

Primers used for generating SOD2 3′UTR luciferase reporter plasmids.

Plasmid 3′UTR fragment position (following stop codon) Forward Primer Reverse Primer
Fragment 1 1–191 5′-GTTTAAACGAGCTCG
CTAGCACCACGATCGTTATGC-3′
5′-CCTGCAGGTCGACTCTAG
ATTTTCAATCACTTGCCC-3′
Fragment 2 1–441 5′-TAAACGAGCTCGCTAGCA
CCACGATCGTTATGCTG-3′
5′-GCCTGCAGGTCGACTCTA
GACGACAAAAAGTGCAATTTTC-3′
Fragment 3 2067–2499 5′-TTAAACGAGCTCGCTAGC
AAGCGGTGTGTGTATGTG-3′
5′-GCCTGCAGGTCGACTCTAGA
CTCTTAAAATCAATCTATC-3′
Fragment 4 2939–3349 5′- TTAAACGAGCTCGCTAGC
TGTTGAGAAATCACAAC-3′
5′- GCCTGCAGGTCGACTCTAGA
CAGTCAAAAACTTCACAC-3′
Fragment 5 1–1662 5′-GTTTAAACGAGCTCGCTA
GCACCACGATCGTTATGC-3′
5′-TGCAGGTCGACTCTAGA
GGCTGAGGTGGGACAATCACTT-3′
Fragment 6 1660–3401 5′-GTTTAAACGAGCTCG
CTAGCTCCTGAATAGCTGGG-3′
5′- CCTGCAGGTCGACTCTAG
AACATGCAAGCATAATGCAG-3′