Skip to main content
. 2022 Apr 14;33(5):892–906. doi: 10.1021/acs.bioconjchem.2c00126

Figure 2.

Figure 2

NOTA-insulin stimulates the insulin signaling pathway in polarized BBB endothelial cell monolayers similar to Humulin. Western blots were performed to assess the expression and phosphorylation of insulin signaling kinases in hCMEC/D3 monolayers following 20 min treatment with or without 100 nM of (A) NOTA-insulin or (B) Humulin. Representative immunoblots and bar charts of the p-AKT/AKT ratio are shown (mean ± standard deviation, SD; n = 3: NOTA-insulin, and n = 6 Humulin). **p < 0.01, ***p < 0.005; unpaired Student’s t-tests.