TABLE 1.
16S rRNA oligonucleotide probes and target groups
| Probe | Target group | Sequence (5′→3′) | Target sitea | Wash temp (°C) | Reference |
|---|---|---|---|---|---|
| 687 | Desulfovibrio spp. | TACGGATTTCACTCCT | 687–702 | 45 | 15 |
| 660 | Desulfobulbus spp. | GAATTCCACTTTCCCCTCTG | 660–679 | 55 | 15 |
| 221 | Desulfobacterium spp. | TGCGCGGACTCATCTTCAAA | 221–240 | 55 | 15 |
| 129 | Desulfobacter spp., D. multivorans | CAGGCTTGAAGGCAGATT | 129–146 | 45 | 15 |
| 814 | Desulfosarcina variabilis, Desulfobotulus sapovorans | ACCTAGTGATCAACGTTT | 814–883 | 45 | 15 |
| 338 | General eubacteria | GCTGCCTCCCGTAGGAGT | 338–355 | 42 | 5 |
| 385 | General SRB | CGGCGTCGCTGCGTCAGG | 385–402 | 45 | 5 |
a
Numbering corresponds to the complementary positions in E. coli 16S rRNA.