TABLE 2.
Estimation of cellular DNA contenta
Probe DNA | Species | Avg cell count (cells ml−1) | Avg DNA yield (fg ml−1) | DNA cell−1 (fg)
|
|
---|---|---|---|---|---|
Actual | Adjustedb | ||||
338 | E. coli | 1.9 × 108 (±0.1 × 108) | 1.0 × 109 (±0.2 × 109) | 5.3 | 6.9 |
660 | D. proprionicus | 7.2 × 107 (±0.8 × 107) | 4.0 × 108 (±0.9 × 108) | 5.6 | 7.3 |
221 | D. autotrophicum | 2.0 × 107 (±0.2 × 107) | 1.0 × 108 (±0.4 × 108) | 5.0 | 6.5 |
687 | D. vulgaris | 2.0 × 108 (±0.4 × 108) | 9.0 × 108 (±0.5 × 108) | 4.5 | 5.9 |
814 | D. multivorans | 4.5 × 108 (±0.5 × 108) | 4.0 × 108 (±0.8 × 109) | 4.3 | 5.6 |
129 | D. postgatei | 6.2 × 107 (±0.3 × 107) | 2.0 × 108 (±0.5 × 108) | 2.4 | 3.1 |
Cell counts and DNA yields were determined with 20-ml log-phase cultures.
Based on extraction efficiency of 70%.