Skip to main content
. 1999 Apr;65(4):1516–1523. doi: 10.1128/aem.65.4.1516-1523.1999

TABLE 2.

Estimation of cellular DNA contenta

Probe DNA Species Avg cell count (cells ml−1) Avg DNA yield (fg ml−1) DNA cell−1 (fg)
Actual Adjustedb
338 E. coli 1.9 × 108 (±0.1 × 108) 1.0 × 109 (±0.2 × 109) 5.3 6.9
660 D. proprionicus 7.2 × 107 (±0.8 × 107) 4.0 × 108 (±0.9 × 108) 5.6 7.3
221 D. autotrophicum 2.0 × 107 (±0.2 × 107) 1.0 × 108 (±0.4 × 108) 5.0 6.5
687 D. vulgaris 2.0 × 108 (±0.4 × 108) 9.0 × 108 (±0.5 × 108) 4.5 5.9
814 D. multivorans 4.5 × 108 (±0.5 × 108) 4.0 × 108 (±0.8 × 109) 4.3 5.6
129 D. postgatei 6.2 × 107 (±0.3 × 107) 2.0 × 108 (±0.5 × 108) 2.4 3.1
a

Cell counts and DNA yields were determined with 20-ml log-phase cultures. 

b

Based on extraction efficiency of 70%.