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Nuclease activity in nine P. putida nuclease integrants. P. putida KT2442 and derivatives with an integrated nuclease gene were grown in E2–10 mM octanoate. Growth medium (top) and chloroform-permeabilized cell fractions representing the periplasm (bottom) were incubated with 4 μg of P. putida KT2442 genomic DNA at 37°C for 1 h. Lanes: 1, MBX926; 2, MBX925; 3, MBX924; 4, MBX923; 5, MBX922; 6, MBX921; 7, MBX920; 8, MBX919; 9, MBX918; 10, P. putida KT2442. chr. DNA, chromosomal DNA.
