FIG. 2.

Analysis of nuclease expression by P. putida KT2442 and P. putida MBX924 grown in a 20-liter fed-batch fermentation. Wild-type (wt) and nuclease-integrated (nuc) Pseudomonas strains were grown as described in the text, and at cell densities of 12 and 35 g/liter, samples were analyzed for nuclease activity (A) and protein (B) in the extracellular growth medium (EC) and periplasm (P). The arrows indicate chromosomal DNA (A) and the putative nuclease (B). Lane 1 contains molecular mass markers (either HindIII-digested λ DNA [A] or a combination of glutamic dehydrogenase [56 kDa], maltose binding protein [43 kDa], triosephosphate isomerase [27 kDa], and trypsin inhibitor [20 kDa] [B]).