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. 1999 Apr;65(4):1524–1529. doi: 10.1128/aem.65.4.1524-1529.1999

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Copyright © 1999, American Society for Microbiology

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FIG. 3 — Nuclease activity in wild-type and transgenic PHA producers with a chromosomally integrated nuclease gene. Chromosomal (chr.) DNA was treated with periplasmic fractions of E. coli MBX245 (lane 1), E. coli MBX988 (::nuc-kan) grown on R medium (lane 2) or Luria-Bertani medium (lane 3), R. eutropha MBX917 (::nuc-kan) (lane 4), R. eutropha NCIMB40124HD (lane 5), Pseudomonas sp. strain MBX985 (::nuc-kan) (lane 6), Pseudomonas sp. strain MBX978 (lane 7), P. putida MBX924 (::nuc-kan) (lane 8), or P. putida KT2442 (lane 9).