TABLE 1.
Strains and plasmids used in this study
| Strain | Relevant characteristics | Reference or source |
|---|---|---|
| P. putida strains | ||
| KT2442 | hsdR; Rifr | 20 |
| MBX918 | KT2442 nuc-kan | This study |
| MBX919 | KT2442 nuc-kan | This study |
| MBX920 | KT2442 nuc-kan | This study |
| MBX921 | KT2442 nuc-kan | This study |
| MBX922 | KT2442 nuc-kan | This study |
| MBX923 | KT2442 nuc-kan | This study |
| MBX924 | KT2442 rrn::nuc-kan | This study |
| MBX925 | KT2442 nuc-kan | This study |
| MBX926 | KT2442 nuc-kan | This study |
| Pseudomonas sp. strains | ||
| MBX978 | MBX strain | |
| MBX979 | MBX978 nuc-kan (high-level nuclease activity) | This study |
| MBX980 | MBX978 nuc-kan | This study |
| MBX981 | MBX978 nuc-kan (low-level nuclease activity) | This study |
| MBX982 | MBX978 nuc-kan (moderate nuclease activity) | This study |
| MBX983 | MBX978 nuc-kan | This study |
| MBX984 | This study | |
| MBX985 | MBX978 nuc-kan (moderate nuclease activity) | This study |
| MBX986 | MBX978 nuc-kan | This study |
| MBX987 | MBX978 nuc-kan | This study |
| R. eutropha strains | ||
| NCIMB40124HD | NCIMBa | |
| MBX917 | NCIMB40124HD nuc-kan | This study |
| E. coli strains | ||
| S17-1 | recA thi pro hsdR−M+ RP4-2-Tc::Mu λpir; Smr | 16 |
| DH5αF− | recA1 hsdR17 endA1 thi-1 gyrA96 relA1 | NEBb |
| MBX245 | LJ14 rpoS::Tn10; Nalr | MBX strain |
| MBX988 | MBX245 nuc-kan | This study |
a
National Center for Industrial Microorganisms and Bacteria.
b
New England Biolabs, Beverly, Mass.