Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 20;22:186. doi: 10.1186/s12883-022-02707-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022, corrected publication 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Stroke patients accumulate pro-inflammatory cytokines and show increased NET formation within the retrieved thrombi. A CD4⁺ T cells of patients with ischemic stroke and control patients were assessed for their intracellular expression of the pro-inflammatory cytokines IL-17, IFN-y and IL-22 and show significantly higher absolute numbers of these T_H1 and T_H17 cytokines in peripheral blood. Data are representative for 35 stroke patients and 16 control patients (Healthy) and presented as mean ± SD. Mann-Whitney-U Test, * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. B Retrieved thrombi of 35 ischemic patients were analyzed via fluorescence microscopy for the expression of intact but activated CD66b⁺ neutrophils within the thrombus and apoptotic neutrophils in form of NET (Sytox Green positive). The MFI for CD66b expression and NET formation within the thrombi were determined. Correlation between these two parameters shows a significant slope for such. C Representative fluorescence images of a thrombus stained for NET (green; left image) and CD66b (red; middle image) as well as an overly of both (merged; right image)