Table 3.

Summary of different gene editing techniques with the main dis/advantages of their use.

Technology	Advantages	Disadvantages	Ref
Lentiviral/retroviral vectors	no insertional oncogenesis reported after several years of use	complicated and slow manufacturing	(83)
Transposons PiggyBack Sleeping Beauty	easy manufacturing, large inserts allowing insertion of multiple CAR constructs, safety switches or other armamentarium	risk of insertional mutagenesis/chromosomal aberrations, CAR-T cell derived lymphomas reported	(84–87)
Targeted mutagenesis TALEN CRISPR/Cas9	targeted gene editing	increased risk of mutagenesis including chromotripsis	(88–91)