Table 4.
The effects of EAAT2-related drugs on AII responses and RB membrane potentials
| Data | Data structure | Type of test | Power | Mean ± SEM | Number of cells |
|
|---|---|---|---|---|---|---|
| Effect of DHK on AII EPSC amplitude (unit: pA) | ||||||
| Control | Normal distribution | −295.40 ± 24.45 | 7 | |||
| 200 μm DHK | Normal distribution | −350.70 ± 36.26 | 7 | |||
| a | Control vs DHK | Paired Student’s t test | p = 0.0111 | 7 | ||
| Effect of GT949 on AII EPSC amplitude (unit: pA) | ||||||
| Control | Normal distribution | −347.50 ± 24.07 | 10 | |||
| 10 μm GT949 | Normal distribution | −274.40 ± 24.73 | 10 | |||
| b | Control vs GT949 | Paired Student’s t test | p = 0.0004 | 10 | ||
| Relative effects of DHK and GT949 on AII EPSC amplitude | ||||||
| Control | Non-normal distribution | 1.00 ± 0.00 | ||||
| 200 μm DHK | Normal distribution | 1.18 ± 0.04 | 7 | |||
| 10 μm GT949 | Normal distribution | 0.78 ± 0.03 | 10 | |||
| c | Control vs DHK | Wilcoxon signed-rank test | p = 0.0313 | 7 | ||
| d | Control vs GT949 | Wilcoxon signed-rank test | p = 0.0020 | 10 | ||
| Effect of DHK on AII EPSC time to peak (unit: ms) | ||||||
| Control | Normal distribution | 12.53 ± 0.44 | 7 | |||
| 200 μm DHK | Normal distribution | 11.87 ± 0.44 | 7 | |||
| e | Control vs DHK | Paired Student’s t test | p = 0.0531 | 7 | ||
| Effect of DHK on AII EPSC rise time (unit: ms) | ||||||
| Control | Normal distribution | 3.98 ± 0.43 | 7 | |||
| 200 μm DHK | Normal distribution | 3.09 ± 0.48 | 7 | |||
| f | Control vs DHK | Paired Student’s t test | p = 0.0050 | 7 | ||
| Effect of DHK on AII EPSC tau (unit: ms) | ||||||
| Control | Normal distribution | 6.73 ± 0.80 | 7 | |||
| 200 μm DHK | Normal distribution | 6.03 ± 0.58 | 7 | |||
| g | Control vs DHK | Paired Student’s t test | p = 0.3519 | 7 | ||
| Effect of DHK on AII mEPSC frequency (unit: Hz) | ||||||
| Control | Normal distribution | 8.25 ± 1.20 | 7 | |||
| 200 μm DHK | Normal distribution | 8.97 ± 1.07 | 7 | |||
| h | Control vs DHK | Paired Student’s t test | p = 0.1120 | 7 | ||
| Effect of DHK on AII mEPSC amplitude (unit: pA) | ||||||
| Control | Normal distribution | −19.47 ± 1.30 | 7 | |||
| 200 μm DHK | Normal distribution | −19.23 ± 0.95 | 7 | |||
| i | Control vs DHK | Paired Student’s t test | p = 0.6892 | 7 | ||
| Effect of DHK on AII mEPSC rise time (unit: ms) | ||||||
| Control | Normal distribution | 0.33 ± 0.01 | 7 | |||
| 200 μm DHK | Non-normal distribution | 0.34 ± 0.00 | 7 | |||
| j | Control vs DHK | Wilcoxon signed-rank test | p = 0.3750 | 7 | ||
| Effect of DHK on AII mEPSC tau (unit: ms) | ||||||
| Control | Non-normal distribution | 0.88 ± 0.06 | 7 | |||
| 200 μm DHK | Normal distribution | 0.90 ± 0.03 | 7 | |||
| k | Control vs DHK | Wilcoxon signed-rank test | p = 0.5781 | 7 | ||
| Effect of DHK on RB light-evoked voltage change (unit: mV) | ||||||
| Control | Normal distribution | 9.62 ± 0.39 | 6 | |||
| 200 μm DHK | Normal distribution | 11.14 ± 0.56 | 6 | |||
| l | Control vs DHK | Paired Student’s t test | p = 0.0036 | 6 | ||
| Effect of DHK on RB resting membrane potential (unit: mV) | ||||||
| Control | Normal distribution | −55.28 ± 1.04 | 6 | |||
| 200 μm DHK | Normal distribution | −57.21 ± 1.76 | 6 | |||
| m | Control vs DHK | Paired Student’s t test | p = 0.1574 | 6 | ||
DHK, a selective EAAT2 blocker; GT949, a positive allosteric modulator of EAAT2; AII, AII amacrine cell; RB, rod bipolar cell.