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. 2022 May 16;17:2203–2224. doi: 10.2147/IJN.S348559

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© 2022 Wanjale et al.

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(A) Histopathological evaluation of paraffin-embedded tumor tissue by hematoxylin and eosin staining, after 6 weeks of treatment. (B) Confocal image-based evaluation by immunofluorescence of proliferation and prognostic marker Ki67 (Alexa 488), counter-stained with GFAP (eFluor 660) positive U251 cells to differentiate mice fibroblasts from human glioma cells and Hoechst 33342 to stain nucleus. (C) Brightfield image-based evaluation of tumor tissue sections stained with TUNEL assay, arrows indicate apoptotic cells with double stranded DNA breaks.