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Uptake of fluorescent dyes by pressure-treated E. coli cells. EB (●) and PI (■) were added to stationary-phase cells of strains C9490 (A), NCTC 12079 (B), and H1071 (C) treated at a pressure of 500 MPa in PBS for different lengths of time. Viable counts (▵) were determined at each measurement time. Fluorescence was expressed as a percentage of the value obtained with cells heated at 80°C for 5 min, which was assumed to be 100%. The curves show the data obtained in single experiments.
