FIG. 1.

(a) Growth and increase in optical density during second-step cultivation of H. pseudoflava on γ-butyrolactone (2 ml/liter) as a sole carbon source in a two-step procedure. Zero time was the beginning of the second-step cultivation. (b) Time course of 4HB monomer incorporation during the second-step cultivation of H. pseudoflava on γ-butyrolactone (2 ml/liter) as a sole carbon source in a two-step procedure. Zero time was the beginning of the second-step cultivation. A660, absorbance at 660 nm.