TABLE 1.
Growth of and polyester accumulation by H. pseudoflava grown on γ-butyrolactone (0.8 ml/liter; 10.4 mM) as the sole carbon source by using the one-step cultivation processa
| Culture time (h) | Dry cell wt (g/liter) | Polyester content (%, wt/wt) | Polyester composition (mol%)b
|
Amt of remaining nitrogen (g/liter)c | Amt of remaining γ-butyrolactone (ml/liter) | C/N ratio | |
|---|---|---|---|---|---|---|---|
| 3HB | 4HB | ||||||
| 120d | 0.69 | 1.3 | 37 | 63 | 0.18 (1.4)e | 0.41 (5.3)e | 3.8f |
| 144 | 0.69 | 6.1 | 45 | 55 | 0.16 (1.2) | 0.35 (4.6) | 3.8 |
| 168 | 0.66 | 8.0 | 44 | 56 | 0.15 (1.1) | 0.34 (4.4) | 4.0 |
| 192 | 0.64 | 7.8 | 45 | 55 | 0.16 (1.2) | 0.33 (4.3) | 3.6 |
a
Two additional sets of batch experiments produced similar results.
b
Calculated from gas chromatographic data.
c
The initial concentration of ammonium sulfate was 0.66 g/liter (5.0 mM).
d
The culture time includes the 96-h induction period.
e
The values in parentheses are molar concentrations.
f
The C/N ratio was defined as the ratio of the molar concentration of γ-butyrolactone to the molar concentration of ammonium sulfate remaining in the medium.