TABLE 2.

Characteristics of the studies included in the systematic review

AUTHOR/YEAR	DERMATOLOGICAL CONDITIONS	TYPE OF STUDY	NUMBER OF PATIENTS	GROUP CONTROL	INTERVENTION GROUP
Davatdarova and Kazimov, 2008	Acne	RTC	72	Standard drug treatment	Autohemotherapy and topical ozone
Gloor and Lipphardt, 1976	Acne	Not RTC	16	NA	Topical ozone
Zeng et al., 2020	Dermatitis	RCT split sides	12	Routine care	Ozonated water immersion and topical ozonated oil
Guizhi et al., 2018	Dermatitis	RTC	60	Common water immersion	Ozonated water immersion and topical ozonated oil application
Jianyun et al., 2018	Dermatitis	RCT split sides	12	Common water wash and common oil application	Ozonated water immersion and ozonated camellia oil
Gao et al.,2019	Psoriasis	Not RCT	20	-	Ozonated oil
Lina et al., 2018	Psoriasis	RCT	40	Methone ointment with 0.2 mg/g of flumetasone	Ozonated oil
Hassaniem et al., 2018	Systemic sclerosis	RCT	50	Drugs (Epilat Retard 40 mg/day)	Drugs (Epilat Retard 40 mg/day) associated with ozone bags
Nowicka, 2017	Systemic sclerosis	Not RCT	42	-	Ozonated water immersion
Jian et al., 2018	Herpes zoster	RTC	60	Routine care: drugs, low-intensity laser, and topical ointment	Routine care associated to ozonated water compress and topical ozonated oil
Kasmawati et al., 2020	Diabetic foot ulcer	RTC	27	Antimicrobial dressings	Antimicrobial dressings associated to ozone bags
Izadi et al., 2019	Diabetic foot ulcer	RTC	100	Routine care (debridement and dressings)	Association of ozone bags and ozonated gel; subcutaneous application around the wound and systemic use by rectal insufflation
Solovâstru et al., 2015	Chronic Venous Ulcers	RTC	29	Routine care (debridement and dressings)	Routine care associated to topical ozonated sunflower oil
Zhang et al., 2014	Diabetic foot ulcer	RTC	50	Routine care (debridement and dressings)	Ozone bag
Wainstein et al., 2011	Diabetic foot ulcer	RTC	32	Routine care (debridement and dressings) and placebo ozone therapy (inactive form)	Routine care associated with ozone bag
Martínez-Sánchez et al., 2005	Diabetic foot ulcer	RTC	51	Only drug therapy	Ozone bag, ozonated oil and rectal insufflation
Campanati et al., 2013	Healing	RTC split sides	30	Topical hyaluronic gel	Ozonated oil

AUTHOR/YEAR	OZONE CONCENTRATION	NUMBER OF SESSIONS/TIME	CONCLUSION	RISK OF BIAS
Davatdarova and Kazimov, 2008	100 mL of blood with 150 mL of ozonated saline solution/topical not reported	Not reported/topical 3–4 times/week	Forms of ozone therapy were effective when compared to drug therapy	Not reported
Gloor and Lipphardt, 1976	Not reported	Once daily for 1 week	Ozone therapy was ineffective against P. acne and other microorganisms	Not reported
Zeng et al., 2020	Ozonated water (3.0 ± 1.5 mg/L); oil not reported	Once daily for 15 minutes and topical oil twice daily for 3 days	Compared to the control group, ozone therapy may significantly mitigate lesions	Not reported
Guizhi et al., 2018	Ozonated water (3.0 ± 1.5 mg/L). Oil not reported	3–5 times/week for 15 min for 2 weeks	Ozone therapy is safe and efficient	Not reported
Jianyun et al., 2018	Ozonated water (3.0 ± 1.5 mg/L). Oil not reported	Twice daily for 7 days	Reduced effects of S. aureus colonization	Not reported
Gao et al.,2019	13.5% ozone	Twice daily for 4 weeks	Ozonated oil may be a safe and efficient treatment	Not reported
Lina et al., 2018	Not reported	Twice daily for 4 weeks	Ozonated oil is safe and effective , equivalent to glucocorticoids topical ointments	Not reported
Hassaniem et al., 2018	Bag: 52 mg/mL of ozone (total volume, 20–50 mL)	Once daily for 30 min for 20 days	Stimulated early wound healing	Not reported
Nowicka, 2017	Ozone at 2.42 mg/min	Twice daily for 10 min for 10 days	Increased joint mobility and skin temperature and decreased its thickness	Not reported
Jian et al., 2018	Ozonated water (3.0 ± 1.5 mg/L); oil not reported	Once daily for 5 days	Reduced pain in herpes-zoster acute phase and shortened virus duration	Not reported
Kasmawati et al., 2020	Bag: 70 μg/mL of ozone	Once every 3 days for 21 days; bag time not reported	Standard treatment combined with ozone therapy reduced the number of bacterial colonies	Not reported
Izadi et al., 2019	Not reported	30 min of ozone bag; topical ozonated oil every 12h; subcutaneous and rectal routes not reported; sessions conducted until complete healing of ulcer: average, 69.44 ± 36.055 days	Efficient as complement to conservative management	Not reported
Solovâstru et al., 2015	15% ozonated sunflower oil (9.6% O3)	Once daily for 30 days	Promising therapeutic option with calming and anti-inflammatory effects	Not reported
Zhang et al., 2014	with 52 μg/ mL ozone (total volume: 20–50 mL)	Bag 30 min once daily for 20 days	Ozone therapy promoted early healing of ulcer	Not reported
Wainstein et al., 2011	Step 1: 96% oxygen and 4% (80 1g/mL) ozone Step 2: 98% oxygen and 2% (40 lg/ mL) ozone.	Step 1: 4 times/week for 4 weeks; step 2: 2 times/week for 12 weeks	Clinical benefit when combined with conservative management	Selection bias; study data collection logistics
Martínez-Sánchez et al., 2005	Bag: 60 mg/L of ozone; oil not reported; systemic use by rectal insufflation: 10 mg ozone and 50 mg/L concentration	Bag: 1 h every day; rectal insufflation for 20 sessions	Therapy considered efficient and may be used as alternative treatment	Not reported
Campanati et al., 2013	Not reported	Once daily for 12 weeks	As effective as hyaluronic acid	Not reported