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. 2022 Apr 7;56(2):185–197. doi: 10.2478/raon-2022-0012

Figure 2.

Figure 2

Vactosertib inhibits TGF-β/Smad signaling. (A) Fluorescence immunohistochemistry assay of p-SMAD2/3 in irradiated primary tumors and lung of 4T1-Luc allografted BALB/c syngeneic mice. Protein expression of p-SMAD2/3 was detected by fluorescence immunohistochemistry (magnification: 20×, scale bar: 50 μm). In confocal images, bright green fluorescence indicated p-SMAD2/3. Representative images are shown from three independent experiments. (B) Protein expression of p-SMAD2/3 was detected in 4T1-Luc cells by western blot analysis. Protein expression was normalized by that of GAPDH.