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. 2022 May 20;8(20):eabm8786. doi: 10.1126/sciadv.abm8786

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Fig. 3. — Cell lines were established with EV or FRAT1 overexpression in NCI-H1299 cells. (A) Whole-cell lysate was subjected to immunoblot analysis with the indicated antibodies. (B) Total RNA was extracted and subjected to RT-qPCR analysis with the indicated primers. (C and D) Cells were fed with U-13C-Serine or U-13C-Glucose for 4 hours, and the metabolites were subsequently extracted and subjected to LC-MS analysis. *P < 0.05, **P < 0.01.