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. 2022 May 20;8(20):eabm8786. doi: 10.1126/sciadv.abm8786

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Fig. 5. — (A and B) NCI-H1299 cells were exposed to 5 mM CHIR99021 for the indicated time, and (A) whole-cell lysate was subjected to immunoblot analysis with the indicated antibodies, or (B) total RNA was extracted and subjected to RT-qPCR analysis with the indicated primers. (C and D) NCI-H1299 cell lines were established with either overexpression of short hairpin green fluorescent protein (shGFP) or dual overexpression of shGSK3a#1 and shGSK3b#1. (C) Whole-cell lysate was subjected to immunoblot analysis with the indicated antibodies, and (D) total RNA was extracted and subjected to RT-qPCR analysis with the indicated primers. *P < 0.05, **P < 0.01.