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. 2022 Feb 19;8(3):1157–1165. doi: 10.1002/vms3.754

TABLE 1.

The primers of this research were designed with Primer Express software

Reference gene Sequence (5′ → 3′) Tm (°C) Product length (bp) Volume
16S ribosomal RNA F: ATTCCACGTGTAGCGGTGAA 60 165 25 μL
R: AGCCCAATCCCCAAATCGAC