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. 2022 May 20;12:8572. doi: 10.1038/s41598-022-12613-7

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Depth of coverage of target regions. The name of each target region represents the gene name and the assigned pedigree ID. The average depth of sequence coverage varied significantly for different PCR amplicons, between 19,626.4 and 127.53 fold. The depth of coverage for PCR amplicons was significantly more uniform within target regions than between, with the average depth per target region varying significantly between 158× and 16418× [ANOVA: F(29, 70) = 10.05; P = 2.446 × 10⁻¹⁵]. Statistical outliers indicated by circles.