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. 2022 May 16;11:e74700. doi: 10.7554/eLife.74700

Figure 3. FBXL12 inhibits KU70/KU80-promoted DNA end resection.

(A) Flow cytometric analysis of chromatin-bound RPA in G0-arrested Lig4-/- abl pre-B cells and Lig4-/-:Xrcc6-/- abl pre-B cells before and 3 hr after 20 Gray IR. Data is representative of three independent experiments in two different cell lines. (B) As in A, in G0-arrested Lig4-/- and Lig4-/-:Fbxl12-/- abl pre-B cells. Data is representative of three independent experiments in at least two different cell lines. (C) Flow cytometric analysis of chromatin-bound RPA in G0-arrested Lig4-/-:Fbxl12-/- abl pre-B cells with and without 10 μM NU7441 treatment, before and 3 hr after 20 Gray IR. Data is representative of three independent experiments in at least two different cell lines (D) Flow cytometric analysis of chromatin-bound RPA in G0-arrested Lig4-/-:Fbxl12-/- abl pre-B cells before and after Xrcc6 knockout, before and 3 hr after 15 Gray IR. Data is representative of three independent experiments.

Figure 3.

Figure 3—figure supplement 1. FBXL12 inhibits KU70/KU80-promoted DNA end resection.

Figure 3—figure supplement 1.

(A) Western blot analysis of KU70 protein in Lig4-/- and Lig4-/-:Xrcc6-/- abl pre-B cells. (B) Western blot analysis of KU70 protein in Lig4-/-:Fbxl12-/- abl pre-B cells.
Figure 3—figure supplement 1—source data 1. Original western blots showing Ku70 depletion in Figure 3—figure supplement 1.
Figure 3—figure supplement 1—source data 2. Original western blots for Ku70 depletion in Fbxl12- cells.