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TNC protein was expressed in neurons, astrocytes, and microglia after focal ischemia. Neurons, astrocytes, and microglia were immunostained with NeuN, GFAP, and TMEM119, respectively. Brain sections were from representative male and female mice of each cohort treated with TNC siRNA or Neg siRNA following 1 h of transient MCAO and 3 days of reperfusion. The scale bar is 50 μm. Similar results were observed in n = 4/group.
