Figure 5.

TNC knockdown prevented post-stroke inflammation. (a) The male TNC siRNA cohort showed reduced infiltration of Ly-6G⁺ microglia/macrophages compared with the Neg siRNA cohort. Similar staining was observed in n = 4/group. Scale bar: 100 μm. (b) The male TNC siRNA cohort showed reduced protein expression of MPO and ICAM-1 compared with the Neg siRNA cohort. (c & d) RNA levels of the inflammatory molecules (IL-6, IL-1β, MCP-1, TNF-α, and TRAIL) were significantly lower in both the male and female TNC siRNA cohorts compared with the Neg siRNA cohorts at 3 days reperfusion following transient MCAO. All parameters were estimated in the peri-infarct cortex at 3 days of reperfusion after transient MCAO. Values are mean ± SD; n = 4/group, *p < 0.05 compared with the Neg siRNA by using the Mann-Whitney U test, and *^/#p < 0.05 compared with the sham/Neg siRNA by one-way ANOVA with Tukey’s multiple comparisons test.