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. 2022 May 10;50(9):4900–4916. doi: 10.1093/nar/gkac347

Figure 3.

Figure 3.

Ψ level in snoRNA targets is modulated by the degree of complementarity between the snoRNA and its target. (A) Heatmap of Ψ-score of synthetic snoRNA targets with increasing complementarity to snoRNAs as measured in HEK-293T cells transfected with Pol-II- or Pol-I-promoter libraries (left and right, respectively). Each row represents constructs targeted by a specific snoRNA (listed on the right). Columns represent the degree of complementarity of the synthetic target to its specific snoRNA. Hierarchical clustering was conducted according to the signals in Pol-II reporters, and reporters in Pol-I were ordered accordingly. N = 2. Missing values are shown in grey. (B, C) Ψ-score values of synthetic snoRNA constructs with increasing complementarity to snoRNAs (B, C) and WT rRNA and snRNA target constructs (C, right group in boxplot) as measured in HEK-293T cells transfected with Pol-II-promoter libraries (n = 6). (D, E) Ψ-score values of synthetic snoRNA constructs with increasing complementarity to snoRNAs (D, E) and WT rRNA and snRNA target constructs (E, right group in boxplot) as measured in HEK-293T cells transfected with Pol-I-promoter libraries (n = 2).