Skip to main content
. 2022 May 7;50(9):5226–5238. doi: 10.1093/nar/gkac315

Figure 4.

Figure 4.

Effects of the 5′-nucleotide of the guide and guide-target mismatches on target cleavage. (A) Preferences for the 5′-nucleotide of the guide. The kobsvalues were determined from the single-exponential fits of the data. Data are the mean ± SD from three independent experiments. (B) P-values for all comparisons of kobs values from (A). nsP > 0.05, compared to the 5′-T guide using Student's t-test. (C) Effects of guide–target mismatches on RNA cleavage by MbpAgo. Data are the mean ± SD from three independent measurements. The reaction was performed with 16 nt 5′P-gDNA at 37°C for 5 min. ***P < 0.001 and ****P < 0.0001, compared to C, the control reactions with guide containing no mismatches, using Student′s t-test. In all experiments, MbpAgo, guide and target were mixed at a 4:2:1 molar ratio (800 nM MbpAgo preloaded with 400 nM guide, plus 200 nM target) and incubated at 37°C.