Figure 7.

Cleavage of highly-structured HIV-1 ΔDIS 5′-UTR RNA by the MbpAgo–gDNA complex. (A) Schematic overview of the MbpAgo-gDNA complex-mediated cleavage assay. Twelve regions (shown in different colors) were selected from the RNA target sequence, with each region targeted by a different 16 nt gDNA. (B) Analyses of the cleavage products obtained after incubation of 5′P-gDNA-MbpAgo complex with HIV-1 ΔDIS 5′-UTR RNA. (C) Analyses of the cleavage products obtained after incubation of the 5′OH-gDNA-MbpAgo complex with HIV-1 ΔDIS 5-′UTR RNA. Experiments in (B) and (C) were carried out with 5 mM Mn²⁺ at 37°C for 30 min. The positions of the targets (T), gDNAs (G) and cleavage products (P) are indicated on the left of the gels. M, RNA marker.