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. 2022 May 7;50(9):5064–5079. doi: 10.1093/nar/gkac316

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Developmental- and pathogen-dependent nuclear accumulation of GFP-APIP5. (A) GFP-APIP5 abundance in cytosolic- and nuclei-enriched fractions from GFP-APIP5 transgenic plants at the seedling and tillering stages. S represents the seedling stage; T represents the tillering stage. Histone H3 served as a nuclear marker and HSP as a cytosolic marker. The experiment was repeated twice (biological replicates) with similar results, and the representative data from one replicate are shown. (B) GFP-APIP5nls abundance in cytosolic- and nuclei-enriched fractions from GFP-APIP5nls transgenic plants at the seedling and tillering stages. S represents the seedling stage; T represents the tillering stage. Histone H3 served as a nuclear marker and HSP as a cytosolic marker. The experiment was repeated twice (biological replicates) with similar results, and the representative data from one replicate are shown. (C) Confocal images showing the subcellular localization of GFP-APIP5 transiently expressed in the leaves of 1- and 2-month-old N. benthamiana plants. mCherry was used a whole-cell localization marker. Scale bars represent 20 μm. (D) GFP-APIP5 abundance in cytosolic- and nuclei-enriched fractions from 1- to 2-month-old N. benthamiana plants. Histone H3 served as a nuclear marker and Actin as a cytosolic marker. The experiment was repeated twice (biological replicates) with similar results, and the representative data from one replicate are shown. (E) GFP-APIP5 abundance in cytosolic- and nuclei-enriched fractions from 3-week-old GFP-APIP5 transgenic plants after inoculation with RO1-1. DAI represents day after inoculation. The experiment was repeated twice (biological replicates) with similar results, and the representative data from one replicate are shown.