Figure 5.

APIP5 functions as a novel RNA-binding protein. (A) Co-localization of GFP-APIP5 with CFP-NbDCP1 and mCherry-NbDCP2 in NPB protoplasts. Scale bars represent 10 μm. (B) Binding affinity of MBP-APIP5 toward poly(A), poly(U) and poly(C). The experiment was repeated three times (biological replicates) with similar results, and the representative data from one replicate are shown. (C) Unlabeled probe competed with the binding activity of MBP-APIP5 with poly(U). Binding activity of MBP-APIP5nls (D) and MBP-APIP5nes (E) with poly(U).