Figure 3.

Assessing the functional consequence of the m¹A9 hypomethylation in 5XFAD. (A) TPM expression levels of mitochondrial mRNAs including those upstream of tRNAs containing m¹A. There are no differences in expression levels in these mRNAs between 5XFAD and WT. (B) Position of primers (in red) flanking the tRNAs that contain m¹A9 sites as detected in this study. (C) RT-qPCR results showing no difference in ‘junction sites’ between 5XFAD and WT. (D) Mature tRNA expression correlates with the observed hypo m¹A9 methylation. (E) No differences are observed in hypomethylated cytosolic tRNA abundances between 5XFAD and WT. ^* denotes a P-value ≤ 0.05 and ^** denotes a P-value ≤ 0.01. Error bars correspond to ±1 SD.